| Product Name | Cat. No. | Specification | Storage Condition | Application |
|---|---|---|---|---|
| Hypotonic MSC Cell Cryopreservation Medium | NC0606 | 100 mL/bottle | Store at 2–8 °C protected from light, 12-month shelf life | Designed exclusively for MSC drug formulations. This GMP-grade hypotonic, serum-free, protein-free and DMSO-free formulation enables stable cell storage at room temperature for 40 minutes, compatible with large-scale manufacturing, filling-line operations and the full workflow of clinical infusion. |
Product Advantages
Well-defined composition with high safety
Serum-free, human-origin-free, animal-origin-free, DMSO-free and protein-free. All components are fully defined to avoid cytotoxicity and risks in clinical application.
Hypotonic osmotic pressure (850–950 mOsm/kg)
Reduces the risk of local tissue edema after infusion and improves safety.
Room-temperature stable, supporting scalable manufacturing
Cells can be kept in the cryopreservation medium at room temperature for 40 minutes, providing a critical time window for production and aliquoting of large-batch cell therapy products.
Ready-to-use, extremely simple operation
Directly usable upon opening.No programmed cooling required. Can be transferred to -80 ℃ freezer directly. Cells can be seeded immediately after thawing. The workflow is simplified with reduced cell loss.
Performance Data
1. Cell Performance
① Umbilical cord MSCs were cryopreserved at three density gradients. Post-thaw recovery rate and cell viability both >90%, with expansion fold >10 within 3–4 days.
| Cryopreservation Density (cell/mL) | 94 h Expansion Fold (Mean) |
|---|---|
| 5E6 | 12.57 |
| 1E7 | 14.1 |
| 2E7 | 14.87 |
② Adipose-derived MSCs were cryopreserved at three density gradients. Post-thaw recovery rate >85%, viability >90%, 24 h attachment rate >100%, with expansion fold >10 within 3–4 days.
| Cryopreservation Density (cell/mL) | 24 h Attachment Rate (Mean) | 78 h Expansion Fold (Mean) |
|---|---|---|
| 5E6 | 128.23% | 10.88 |
| 1E7 | 146.01% | 10.37 |
| 2E7 | 146.87% | 10.04 |
The data demonstrate that this cryopreservation medium maintains high post-thaw recovery and viability for mesenchymal stem cells from different sources, and effectively preserves their proliferative capacity.
2. Cryopreservation Stability
| Cell Type | Cryopreservation Duration | 24 h Attachment Rate (Mean) | 3–4 d Expansion Fold (Mean) |
|---|---|---|---|
| Umbilical cord MSC | 7 days | 115.13% | 12.58 |
| Umbilical cord MSC | 30 days | 121.93% | 12.12 |
| Adipose MSC | 7 days | 140.37% | 10.43 |
| Adipose MSC | 30 days | 160.58% | 12.38 |
The data show that for both short-term (days) and long-term (up to 1 month) cryopreservation, this medium sustains high recovery and viability of MSCs from different origins without impairing cell proliferation.
